Proposed Research Projects

(Note that not all research projects and groups are available each summer. The list found here is to give a general idea of the program’s offering.)


Polymer Based Vesicles for Therapeutics
Dr. Douglas Adamson (Polymer Chemistry)

PolymersomesEfficient, yet extremely inexpensive, porous electrodes can be used for applications from super capacitors to the removal of salt from water. The enabling technology for these electrodes is based on our recent discovery that graphite can be exfoliated into individual graphene sheets without the application of harsh chemical treatments or high energy mixing. This process relies on the graphene sheet’s ability to act as a surfactant, and as such, these sheets have been shown to stabilize water-in-oil emulsions. By choosing the appropriate oil, these emulsions can be cured to create strong and electrically conductive porous materials, and preliminary data has shown them to be efficient in the removal of salt from water by a process termed “Capacitive Deionization” or CDI. The REU student for this project will be involved in both the synthesis of new graphene based electrodes as well as the testing and design of new devices. For a more detailed description of the approach, please see our recent publications as listed on our group website.
Adamson Group Web Site



Mechanistic Inorganic Chemistry
Dr. Alfredo Angeles-Boza (Inorganic Chemistry)

The increasing emission of greenhouse gases, in particular carbon dioxide, has received considerable attention due to its serious environmental consequences. An obvious solution is the capture and storage of the CO2 produced in the industrial processes. A more attractive approach is to combine the capture of CO2 with its use as a renewable and environmentally friendly resource. For example, CO2 can be converted to C1 feedstock for liquid fuels. With this objective in mind, we have recently begun to explore rhenium complexes as (pre)catalysts for the reduction of CO2 to CO. The REU student working on this project will synthesize and characterize heterocyclic ligands and rhenium complexes. The student will also explore the catalytic activity of the synthesized complexes.

Our group is also interested in studying the mechanisms by which host defense peptides use metal ions to enhance their antimicrobial activities. By learning how these important effectors of the immune system interact will lead us to the design of new antibiotics. The REU student working on this project will synthesize peptides or peptide mimics and perform biochemical and biophysical assays to determine their antimicrobial activities.
Angeles-Boza Group Website




Use of Persistent Radical Catalysts in Living Polymerization Reactions
Dr. Alexandru Asandei (Polymer Chemistry)

Red1Our group is interested in complex unconventional organic and polymer syntheses, new concepts, reactions and mechanisms. This includes radical polymerizations, polycondensations, liquid crystals, auxetic materials, self assembly and nanostructures. Molecular weight and polydispersity control in living radical polymerization (LRP) is based on the persistent radical effect (PRE). Persistent radicals are not involved in homodimerization reactions or addition to the double bond, and maintain radical concentration at low levels via their reversible end-capping with the growing chain. Various carbon or oxygen centered radicals (nitroxides, addition fragmentation derivatives) and metaloradicals (Cu, Ni, Fe, Ru complexes) are usually employed in LRP. Novel organic and inorganic systems capable of accomplishing LRP are currently being explored. One goal is the design, synthesis and characterization of catalysts capable of dual or multiple mechanism-polymerizations. The REU student will be involved in the synthesis of monomers, catalysts and polymers and in their characterization. He will acquire experience in organic, inorganic and polymer chemistry and will become familiar with instruments such as NMR, GPC, and DSC.
Asandei Group Web Site



Synthesis and Study of DNA Damages
Dr. Ashis K. Basu (Bioorganic Chemistry)

We study chemicals and drugs that exert their biological effects through DNA damage. Some of the chemicals are environmental pollutants such as 1–nitropyrene. We also study ionizing radiation-induced DNA damages. The REU student will synthesize a specific DNA damage such as a DNA adduct of a nitroaromatic compound or induce an ionizing radiation damage into a designed oligo¬deoxy¬nucleotide. These DNA lesions can induce mutations which may represent the first step converting a normal cell into a cancer cell. Our goal is to correlate the type of mutation with three dimensional architectural effects induced in DNA. The modified DNA fragments will be used to study mutagenesis and DNA repair.
The project will introduce the REU student to a variety of organic synthesis and nucleic acid chemistry tools, chromatography, and structural characterization (NMR, UV-Vis, MS), and introduce the student to molecular biology and recombinant DNA techniques.
Basu Web Site


Synthesis of Pyrrole-Modified Porphyrins
Dr. Christian Brueckner (Organic Chemistry)

Photodynamic therapy (PDT) employs the combination of a photosensitizer, such as a porphyrin, and light to destroy diseased cells. For PDT to be most effective, the light that activates the drug must penetrate deep into tissue. However, while tissue is only transparent for red and infrared light, porphyrins cannot be activated using red light. Thus, our group has set out a program to modify synthetic porphyrins in a way that they can become photosensitizers which can be activated with red light. Although porphyrins are ubiquitous naturally occurring macrocycles, the regio-selective modification of them can be difficult. Hence, synthetic compounds are needed.

We modify a class sof symmetric meso-aryl-substituted porphyrins by formally replacing one pyrrole by a different heterocycle. One reaction sequence involves the cleavage of the ß,ß’-bond (1 to 2), followed by ring-closure to, in this example, form morpholine-derived porphyrin 3. Oxazole-, imidazole, and pyrazole-based systems are also available along this route.


The REU student will do multi-step syntheses (1-4 steps), purification (column and preparative thin layer chromatography) and characterization (UV-vis, IR, fluorescence spectroscopy, NMR) of porphyrins and metalloporphyrins (NiII, ZnII, AgII). The student will learn many analytic and synthetic techniques employed in modern organic and coordination chemistry.
Brueckner Group Web Site

Modeling the Mechanisms of Light Harvesting in a Photosynthetic Antenna Protein
Dr. Jose Gascon (Physical and Computational Chemistry)

In Prof. Gascon’s group you will carry out multi-scale computer simulations to investigate the first events during light harvesting by carotenoids in a protein antenna. Such levels of molecular detail will allow us to correlate structural properties with spectroscopy analysis. This area of research is of fundamental relevance to socially important topics such as the development of alternative solar energy conversion schemes. To utilize energy from sunlight efficiently for photocatalysis or to produce fuels, we first must learn how nature capture photons especially in the mid-visible regime and transfer the captured energy to reactive sites. You will learn the fundamentals of various levels of theory, from Molecular Mechanics to Quantum Mechanics. If you are interested in chemistry and computation, this experience will challenge you and broaden your understanding of the importance of computational modeling in today’s scientific endeavors.

Gascon Group Web Site


Hybrid Materials
Dr. Jie (Jay) He (Polymer Chemistry and Physical Chemistry)

Amphiphilic molecules such as liquids, surfactants, and amphiphilic block copolymers can spontaneously form a wide range of nano- or microstructures such as spherical micelles, cylindrical or worm-like micelles, or bilayer vesicles in selective solvents. Analogues to the self-assembly behaviors of atoms or molecules, the self-assembly of colloidal building blocks,so-called “colloidal molecules”, into various supra-architectures or ordered ensembles provides new opportunities to engineering structures and devices with unique optical, magnetic, or electronic properties. Our group is interested in design and synthesis of colloidal molecules and the use of colloidal molecules as model systems to understand atomic or molecular interactions in self-assembly or crystallization.
The REU student will be trained with various living polymerization techniques (ATRP and RAFT polymerization) and characterization tools (NMR, GPC and electronic microscopes). The student will be exposed to the synthesis and self-assembly of various nanomaterials.


He Group Web Site


Synthesis of Novel Nucleosides
Dr. Amy R. Howell (Organic Chemistry)

Strained oxygen heterocycles, such as epoxides, play a role as biologically significant entities, as well as being versatile intermediates in the syntheses of a variety of molecules. Recently, our group has developed the first general synthesis of 1,5-dioxaspiro[3.2]¬hexanes 1, an unusual and little explored class of compounds. We discovered that dioxaspirohexanes display a fascinating dichotomy of reactivity, providing either highly functionalized ketones 2 or substituted oxetanes 3. We have recognized that with the proper nucleophile we can access novel nucleoside analogs 4. Nucleosides continue to be key therapeutic agents for the treatment of viral diseases, such as HIV and hepatitis B, and of certain types of cancer.


The REU student will prepare appropriately substituted dioxaspiro¬hexanes by well-established protocols and explore conditions for the efficient conversion of these compounds to novel nucleoside analogs. The student will routinely use chromatographic (GC, TLC, column chromatography) and spectroscopic (NMR, IR, GC/MS) techniques for the purification and identification of intermediates and products.
Howell Group Web Site



Shape-Memory Polymers
Dr. Rajeswari M. Kasi (Polymer Chemistry)

Shape memory behavior of an amphiphilic polymer with a triggering T of 25 to 40°C
Shape memory behavior of an amphiphilic polymer with a triggering T of 25 to 40°C

We seek to synthesize, characterize, and, thereby, achieve a fundamental understanding of new biocompatible stimuli-responsive polymers. Development of new synthetic methodologies, modification of existing synthetic routes, multidisciplinary approach to structure-property evaluation, and advanced characterization tools are the overriding factors to rational material design.
Shape memory polymers are a class of responsive polymers that show a reversible temporary shape change with temperature. Upon temperature reduction the initial or permanent shape is achieved once again. We are interested in exploring the influence of architecture and states of matter on shape memory application. The triggering temperature used for these applications could be the glass transition, melting or liquid crystalline transition temperature leading to a multi-variable shape memory approach, Figure 1. Shape memory polymers and hybrid structures can be used in drug delivery, tissue engineering scaffolds, artificial muscles, and actuators.

The undergraduate student researcher will be mentored by a graduate student and the faculty member. The student will learn synthetic polymer chemistry methods and characterization techniques to investigate stimuli-responsive and shape memory properties.
Kasi Group Web Site



Biocatalytic Nanomaterials
Dr. C. Vijaya Kumar (Biophysical)

Our group is interested in synthesizing novel nanomaterials and use them to enhance the biological activities of enzymes, proteins and nucleic acids by rational approaches. In this program, the REU student will learn synthesis of nanomaterials by a very simple approach developed in our laboratories and use the materials for the nanoencapsulation of enzymes. The encapsulated enzymes will be tested for biological activities under extreme conditions, such as organic solvents, high temperatures, and extreme pHs. Nanoparticle-bound enzymes are finding extensive applications in medical diagnostics, organic synthesis, cancer therapy, and environmental remediation. For example, we are using nanoparticle-bound enzymes for the conversion of carbon dioxide into useful chemical substances. The student will gain experience in organic and inorganic synthesis as well as in spectroscopic methods and the activity evaluation of enzymes.
Kumar Group Web Site


From the Kitchen to the Lab
Dr. Nicholas Leadbeater (Inorganic Chemistry)

We all know that microwave ovens can be used for heating food fast. An exciting area of study in the synthetic chemistry community is the use of microwaves for making molecules rapidly, easily and cleanly. Using microwave heating, it is possible to enhance the rate of chemical reactions significantly and to do chemistry that was otherwise not possible. Unlike the microwave at home, we use state-of-the-art scientific microwave systems that allow precise control of reaction conditions. One limitation at the moment is the scale-up of reactions to make multi-gram or kilo quantities of compounds. However, we are about to receive a microwave apparatus that is designed to overcome this hurdle. As an REU student, you would play an important role in using this apparatus over the summer and would have your own mini-project focused around the use of microwave heating for scaling-up reactions. You will be mentored by a graduate student in the group. The reactions will be performed in water as a solvent rather than organic solvents thus making the chemistry more environmentally friendly. As well as being exciting, the project will introduce you to a range of modern synthetic chemistry techniques as well as analysis methods.
Leadbeater Group Web Site


Supramolecular Assembly of Polypeptides into Nanomaterials
Dr. Yao Lin (Polymer Chemistry)

research-yaoOur research interests are to establish nanomaterial platforms that will bring in interesting biological characteristics (e.g. self assembly and hierarchical assembly) for technology applications. In this specific project, the students will work on the supramolecular polymerization of synthetic polypeptides (functionalized homopolymer, block or random copolymers, or brush-like complex polymers) in solution. The aim is to exploit the design principles and applications of complex macromolecules with intrinsic secondary structures components (e.g. alpha helices). Recently, we have successfully polymerized polypeptide-grafted brush-like polymers into giant superhelical structures (as shown in the transmission electronic microcopy image in the figure). We are now pursuing the general, rational design of polypeptide-based polymers that can be used as macromolecular monomers for supramolecular polymerization into other giant nanostructures. The students will work closely with the PI, the postdoc and the graduate students, and have plenty of opportunities to learn and use state-of-art synthesis and characterization methods available in the Chemistry department and at the Institute of Materials Science.
Lin Group Web Site




Control of Photo-Generated Charge-Separated States in Donor-Bridge-Acceptor Molecules
Dr. Tomoyasu Mani (Physical Chemistry)

Research in the Mani Group focuses on photo- and radiation-induced fundamental chemical reactions in the condensed phase. We are particularly interested in controlling electronic excited states, charge and exciton transfer reactions, and spin dynamics in molecules and molecular assemblies. The fundamental understanding of these phenomena will help us improve and develop energy and biomedical technologies.

The REU student will work on the projects that examine the way(s) to control photo-generated charge-separated states. Students will have an opportunity to do either or both organic synthesis and optical (both steady-state and time-resolved) spectroscopy experiments.

Mani Group Web Site


Nanoscale Controlled Light Emitting Devices by Self-Assembly Techniques
Dr. Fotios Papadimitrakopoulos (Polymer Chemistry)

Implantable biosensors could be a plausible way to continuously monitor blood glucose levels, provided they exhibit long-term stability and means to establish telemetry. However, their potential applications remain largely unexploited due to the negative tissue responses such as biofouling, inflammation, tissue fibrosis, and calcification generated by the implantation of such devices. Other problems such as electrical short, signal drifts and need for continuous calibration can lead to device malfunctioning and eventually failure. Also, one of the chief concerns is the possibility of sensor breakdown because of oxidative degradation of enzyme and other electrode coatings due to excess of hydrogen peroxide present in the immediate vicinity of the sensing electrode. This is a direct result of over-sampling of the glucose in the blood stream. Coating the device by a biocompatible, semipermeable membrane can rectify this situation. Apart from acting as a barrier to permeation of glucose, the membrane would protect the sensor from foreign molecules that cause fouling.
Our group investigated the simplistic, yet versatile approach of layer-by-layer (LBL) self-assembly of assembly of Humic Acids (Has), a naturally occurring biopolymer and Fe3+ cations. Not only did these coatings provide the required degree of glucose permeability, but in vivo results indicated their biocompatibility with reduced tissue fibrosis upon implantation. Furthermore, the conformation and growth characteristics of the HAs/Fe3+ membrane could be tailored by carefully adjusting the pH of the aqueous medium. Apart from the HAs/Fe3+ bilayers, we self-assembled films of HAs/poly (diallyldimethylammonium chloride) (PDDA) and also films of poly (styrene sulfonate) (PSS)/PDDA onto the sensory device. Moreover the diffusion coefficients of glucose through these membrane systems were investigated in order to explain the individual sensor response as it pertains to the microstructure of these outer semipermeable membranes. The hysterisis behavior of these sensors was studied as a function of permeability of the outer membrane. It was concluded that the microstructure of these coatings govern the permeability of glucose and correspondingly, the sensitivity, longevity and hysterisis of the sensors.
We plan to extend this outer membrane research to a more biocompatible polyelectrolytes like poly saccharides and proteins, which we aniticipate to finish within one summer.

The incoming REU student will be exposed to a variety of techniques including electrochemical sensor fabrication, electro-analytical techniques, ellipsometry, enzyme immobilization, electropolymerization of conducting polymers, layer by layer assembly, in vitro and in vivo testing of electrochemical sensors as well diffusional based theoretical modeling of electrochemical sensors.
Papadim. Group Web Site


Synthesis as a Tool in Glycoscience
Dr. Mark W. Peczuh (Organic Chemistry)

Carbohydrates are indispensable to biological processes such as metabolism, protein folding, and cell-cell interactions. Our group is interested in the design, synthesis, and characterization (conformation, binding) of ring expanded carbohydrates that can interact with natural proteins such as lectins and glycosidases. The preparation of novel ligands of these two broad groups of carbohydrate binding proteins may provide new tools for glycobiology or even future drug leads.

The REU student will synthesize septanose carbohydrate glycosides and glycoconjugates designed for their ability to bind natural lectins and glycosidases. The routes for their synthesis will rely on established procedures, or will be developed by the student. They will be multistep sequences (4-6 steps), where compound purification (chromatography, crystallization) and spectroscopic characterization (NMR, IR, CD, MS) are critical aspects of the research.
Peczuh Group Web Site




Building Functional Nanodevices with Porous Nanocapsules
Dr. Eugene Pinkhassik (Materials/Organic/Analytical/Nanoscience)

Our research group designs functional nanomaterials and devices with new and superior properties to address global challenges in energy-related technologies, sensing, and medical imaging and treatment. We have developed a directed assembly method for the synthesis of vesicle-templated nanocapsules. These nanocapsules offer a unique combination of properties enabled by robust shells with the single-nanometer thickness containing programmed uniform pores capable of fast and selective mass transfer. Vesicle-templated nanocapsules emerged as a versatile platform for creating functional devices, such as nanoreactors, nanosensors, and containers for drug delivery.

The REU student will learn an array of synthetic and analytical techniques ranging from the synthesis of polymer nanocapsules, using self-assembled structures to direct organic synthesis, characterizing nanoscale objects with light scattering and electron microscopy, and evaluating the performance of newly created nanodevices with spectroscopic and chromatographic methods. Having mastered the synthesis of nanocapsules, the REU student will use the capsules to build nanodevices aiming at one of the following applications: nanoreactors with encapsulated homogeneous or enzymatic catalysts, highly selective nanoprobes, containers for the delivery of drugs or imaging agents, or cell-mimicking devices capable of through-shell communication.

Pinkhassik Group Web site


Reliability and Engineering of Molecules and Materials Next-generation Electronics.
Dr. Rebecca Quardokus (physical/materials/nanoscience)

The Quardokus group focuses on the reliability and engineering of molecules and new materials for next-generation electronics. Scanning tunneling microscopy (STM), with its ability to image individual atoms and molecules, is the primary tool used to investigate surface-confined molecular interactions and two-dimensional materials.  The systems of interest include self-assembled monolayers, two-dimensional polymers, surface-confined reactions, hierarchical designs, and surface-confined molecular rotors and switches.
Quardokus Group Web Site


Enzyme-assembled Nanocapsules for Targeted Drug Delivery
Dr. Jessica Rouge (Biological Chemistry)

We seek to design, synthesize and characterize nanomaterials that can target specific cell types for the delivery of therapeutic nucleic acids and small molecule drugs.

Nanomaterials have revolutionized the way drugs can be delivered thanks to their small size and enhanced chemical stability. However the ability to direct them to specific cellular targets and to control the release of their therapeutic cargo has been a major obstacle in the field. Our lab seeks to develop new materials that can direct the localization of a nanomaterial to specific cell receptors through the use of DNA aptamers. Aptamers are DNA and RNA sequences that strongly bind specific cellular locations or proteins. We are also interested in controlling the release of the nanomaterials contents through interactions with specific enzymes (esterases). We work to synthesize new substrates that can direct enzymes to the surface of nanomaterials in order to facilitate the enzyme-mediated assembly of chemically modified aptamers to particle surfaces along with the degradation of the nanomaterials itself.

An undergraduate researcher will be exposed to a highly interdisciplinary lab environment, being trained by both a graduate student and the faculty member. The students will learn both chemical and biochemical techniques such as nanoparticle synthesis, automated DNA synthesis, HPLC, PCR, RNA transcription and other enzymatic reactions.
Rouge Group Web Site

Cancer Biomarker Detection by Immunoarrays
Dr. James F. Rusling (Analytical, Physical Chemistry)

immunoarrayOne focus of our research is to design immunoarrays for proteins based on nanomaterials. This project is in collaboration with materials scientist Profs. Papadimitrakopoulos at UConn and the NIH cancer biologists in Bethesda, MD, and has early cancer detection and monitoring as its goal. In this project, the REU student will utilize newly developed array fabrication and coating techniques to develop and evaluate electrochemical immunoarrays based on gold nanoparticle electrodes in microfluidic devices. Arrays will utilize captured antibodies spotted on multi-electrode chips to detect major protein biomarkers for oral and other cancers.

The student will develop analytical protocols for these analyses in serum samples, and attempt to improve sensitivity, detection limit and reproducibility compared to our existing arrays. The student will learn state-of-the-art biomedical sensor preparation technology utilizing nanoparticles and ink-jet biomolecule spotting. The student will also gain experience in electrochemical, AFM and spectroscopic analyses to monitor array fabrication, and the use amperometry for biomarker detection with the microfluidic arrays.
Rusling Group Web Site



Surface Initiated Polymerization on Inorganic Oxides
Dr. Thomas A.P. Seery (Polymer Chemistry)

Nanostructured materials have generated intense interest in recent years. The ability to control molecular structure on a scale intermediate between organic molecules and bulk matter has become increasingly important in technologies relying on thin organic films. Our research has advanced the concept of surface initiated polymerization (SIP) as a means to prepare conformal polymer monolayers on hard substrates. Gold and silicon oxide surfaces have been used as model systems to demonstrate this concept. Idealization of SIP consists of the following steps: (1.) Surface coverage of initiator is formed. (2.) Active catalyst is generated. (3.) Polymerization via chain growth mechanism provides polymer with catalyst on the living ends. (4.) Endcapping gives control over ultimate surface properties.

The REU student working on this project would lead the effort to extend this concept to titanium oxide surfaces. This is a fairly challenging task, but the student would be working alongside an interdisciplinary team of experienced graduate students and postdoctorals. The first task would involve functionalizing the titanium surface with monochlorosilane coupling agents that have terminal vinyl groups. The next step is detection of these species at the surface using ellipsometry and contact angle measurements. The vinyl species will be reacted with ruthenium alkylidene complexes to form catalytic species on the surface. Polymerization then occurs after addition of strained cyclic olefins. This project is modular, and the interdisciplinary nature will allow a student the freedom to focus on either synthetic or physical chemistry.
Seery Web Site


Catalysts, Ceramics, Batteries, and Adsorbents
Dr. Steven L. Suib (Inorganic Chemistry)

Departments of Chemistry, Chemical Engineering, and Materials Science and Engineering, and Institute of Materials Science.

Our NSF funded research program involves the preparation of aligned crystallites on solid surfaces that can be used as Catalysts, Ceramics, Batteries, and Adsorbents. Much of this research involves synthesis of novel metal oxide and sulfide materials that are densely packed but accessible to chemical reagents for distinct chemical and physical reactions.


Figure 1, Diagram of Oriented Fibers; Synthesis, Scanning Electron Micrograph and Coated Product.

Figure 1 above shows a diagram of one of the synthetic processes that is used to make such oriented crystallites. These nano-sized materials are shown to be well aligned in the scanning electron micrograph shown above. The photograph on the right in Figure 1 is that of an uncoated (cream color) cordierite monolith like that in an auto exhaust system in cars and the coated (dark brown) honeycomb support with aligned crystallites. A major advantage of the alignment is that more accessible sites are available for whatever the specific application might be.

For example, the materials in Figure 1 are being studied as auto exhaust catalysts and have shown excellent activity and stability in the oxidation of CO and the reduction of NOx. the same types of oriented materials can enhance the capacity of battery materials, and increase the amount of adsorption for example of extracting harmful sulfur and nitrogen species from a variety of fuels. Many of these materials act as ceramic systems that are stable at very high (> 500oC) temperatures.

The type of research that would be done under an REU summer program would involve any aspect of synthesis, characterization, or applications of such oriented materials. Related goals of this research program involve use of green reagents, regeneration and sustainability of systems, and scale-up of materials and processes.


Chen, S. Y.; Song, W.; Lin, H. J.; Wang, S.; Biswas, S.; Mollahosseini, M.; Kuo, C. H.; Gao, P. X.; Suib, S. L.; Manganese Oxide Nano-Array Based Monolithic Catalysts: Tunable Morphology and High Efficiency for CO Oxidation, ACS Appl. Mat.  & Int., 2016, 8, 7834-7842.

Dutta, B.; Biswas, S.; Sharma, V.; Savage, N. O.; Alpay, S. P.; Suib, S. L., Mesoporous Manganese Oxide Catalyzed Aerobic Oxidative Coupling of Anilines to Aromatic Azo Compounds, Ang. Chem. Int. Ed., 2016, 55, 2171-2175.
Suib Group Web Site


Synthesis of stable organic mono- and diradicals
Dr. Gaël Ung (Inorganic/organic)

Radical species are typically highly reactive because of the presence of an unpaired electron. Our group specializes in taming reactive species and the study of unusual species. This project aims at preparing boron containing mono- and diradicals stabilized by extended conjugation. It is anticipated that these molecules will possess unusual optical and magnetic properties, thus paving the way to the development of luminescent or conductive organic materials.


The REU student will learn basic organic synthesis techniques as well as advanced inorganic synthetic techniques for the handling of air- and moisture sensitive compounds (Schlenk techniques, glove box). The student will also be exposed to a variety of spectroscopic methods for the characterization of the synthesized compounds.

Ung Group Web Site


Mass Spectrometry to Investigate Micro-Scale Preparation of Peptide Samples
Dr. Xudong Yao (Analytical Chemistry and Biological Chemistry)

Mass spectrometry is used as a fast and sensitive tool to study peptides. Mass spectrometry analyzes charge-to-mass ratios of peptide ions in gas phase. A mass spectrum plots the intensities of ions against their charge-to-mass ratios. These ratios can be used to determine chemical structures of peptides, while the intensities give relative quantitation of the ions. Sample preparation of peptides is a key step for successful mass spectrometric analysis, and it is often done at a micro-scale. In REU summer projects, students will work on different sample manipulations of peptides such as chemical modification of peptide mixtures and use mass spectrometry to study the efficiency of various micro-scale procedures for peptide sample preparation.
The REU project will specifically investigate analytical challenges in mass spectrometric analysis of phosphopeptides. Phosphopeptides are fragments of phosphoproteins that are important regulators for cellular signaling. Analysis of protein phosphorylation is important to understand and treat various human diseases and to manipulate the fate of stem cells for therapeutic and regenerative applications. The REU researcher will study ß-elimination and Michael addition reactions of phosphopeptides. Objectives of the project are to minimize side reactions and maximize the efficiency of the sample preparation workflow that will be examined by high performance liquid chromatography and tandem mass spectrometry.
Yao Group Web Site